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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS

Altyn Khassein 1 ORCID , Botakoz Tezekbayeva 1 ORCID , Karlygash Aubakirova 2 ORCID , Gulnur Suleimanova 1 ORCID , Natalya Malakhova 2 ORCID

1 NJSC "Kazakh National Agrarian Research University"; 2 M.A. Aitkhozhin’s Institute of Molecular Biology and Biochemistry

doi.org/10.37884/03-1-2025/02 Admitted 11.07.2025 Published 15.10.2025

Abstract

This paper presents the results of using complex biotechnological methods for in vitro treatment of 16 grape varieties (Vitis vinifera L.) against viral infections. The drug "Kagocel" was used as an antiviral agent in concentrations of 10 and 30 mg/l, both in pure form and in combination with thermotherapy. Diagnostics of plant material for the presence of viral pathogens was carried out using the enzyme-linked immunosorbent assay (ELISA) before and after treatment. For shoot growth and development and micropropagation of plants, half-strength MS (1/2) medium containing vitamins, iron chelate, supplemented with 30 g/l sucrose and 6.3 g/l agar, without plant growth regulators was used; callus formation, hyperhidrosis or necrosis of shoot tips were not observed. It was found that the efficiency of eliminating raspberry bushy dwarf virus (RBDV) significantly increases with combined treatment - chemotherapy (drug «Kagocel») and in vitro thermotherapy (36-38 °C for 50 days). Elimination of RBDV in variants K10 + T and K30 + T was 77.2% and 79.8%, respectively.

grape Vitis vinifera L in vitro virus elimination thermotherapy chemotherapy

01 Introduction

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02 References

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Citation Links

[1]Khassein , A. et al. 2025. STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister natigeler. 03-1(special) (Oct. 2025). DOI:https://doi.org/10.37884/03-1-2025/02.
(1)Khassein , A.; Tezekbayeva , B.; Aubakirova , K.; Suleimanova , G.; Malakhova , N. STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister natigeler 2025, No. 03-1(special). https://doi.org/10.37884/03-1-2025/02.
Khassein , A., Tezekbayeva , B., Aubakirova , K., Suleimanova , G., & Malakhova , N. (2025). STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister Natigeler, 03-1(special). https://doi.org/10.37884/03-1-2025/02
KHASSEIN , Altyn; TEZEKBAYEVA , Botakoz; AUBAKIROVA , Karlygash; SULEIMANOVA , Gulnur; MALAKHOVA , Natalya. STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister natigeler, [S. l.], n. 03-1(special), 2025. DOI: 10.37884/03-1-2025/02. Disponível em: https://journal.kaznaru.edu.kz/index.php/research/article/view/983. Acesso em: 17 oct. 2026.
Khassein , Altyn, Botakoz Tezekbayeva, Karlygash Aubakirova, Gulnur Suleimanova, and Natalya Malakhova. 2025. “STUDY OF THE EFFECTIVENESS OF THE DRUG ‘KAGOCEL’ AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS”. Izdenister Natigeler, no. 03-1(special) (October). https://doi.org/10.37884/03-1-2025/02.
Khassein , A. (2025) “STUDY OF THE EFFECTIVENESS OF THE DRUG ‘KAGOCEL’ AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS”, Izdenister natigeler [Preprint], (03-1(special). doi:10.37884/03-1-2025/02.
[1]A. Khassein, B. Tezekbayeva, K. Aubakirova, G. Suleimanova, and N. Malakhova, “STUDY OF THE EFFECTIVENESS OF THE DRUG ‘KAGOCEL’ AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS”, Izdenister natigeler, no. 03-1, Oct. 2025, doi: 10.37884/03-1-2025/02.
Khassein , Altyn, et al. “STUDY OF THE EFFECTIVENESS OF THE DRUG ‘KAGOCEL’ AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS”. Izdenister Natigeler, no. 03-1(special), Oct. 2025, https://doi.org/10.37884/03-1-2025/02.
Khassein , Altyn, Botakoz Tezekbayeva, Karlygash Aubakirova, Gulnur Suleimanova, and Natalya Malakhova. “STUDY OF THE EFFECTIVENESS OF THE DRUG ‘KAGOCEL’ AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS”. Izdenister natigeler, no. 03-1(special) (October 15, 2025). Accessed October 17, 2026. https://journal.kaznaru.edu.kz/index.php/research/article/view/983.
1.Khassein A, Tezekbayeva B, Aubakirova K, Suleimanova G, Malakhova N. STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister natigeler [Internet]. 2025 Oct. 15 [cited 2026 Oct. 17];(03-1(special). Available from: https://journal.kaznaru.edu.kz/index.php/research/article/view/983
1.Khassein A, Tezekbayeva B, Aubakirova K, Suleimanova G, Malakhova N. STUDY OF THE EFFECTIVENESS OF THE DRUG "KAGOCEL" AND THERMOTHERAPY IN VITRO FOR THE TREATMENT OF GRAPES FROM THE RASPBERRY BUSHY DWARF VIRUS. Izdenister natigeler. 2025;(03-1(special). doi:10.37884/03-1-2025/02