ДНҚ МАРКЕРЛЕРМЕН ҚАЗАҚСТАНДЫҚ СЕЛЕКЦИЯНЫҢ ЖҮЗІМ СОРТТАРЫ МЕН ГИБРИДТЕРІНІҢ МИЛДЬЮ АУРУЫНА (Plasmopara viticola) ТӨЗІМДІ ГЕНДЕРІН АНЫҚТАУ

Лаура Ажитаева; Карлыгаш Аубакирова; Сауле Казыбаева; Сәуле Қорабаева; Асылбек Айтенов; Лаура Ерболова

doi:10.37884/4-2024/24

Abstract

Grapes are an important fruit crop in Kazakhstan. Downy mildew is one of the most common fungal diseases of grapes caused by Plasmopara viticola. An effective way to control the spread of the pathogen is the cultivation of resistant varieties. Vitis Vinifera varieties are considered the basis of high-quality viticulture, but are susceptible to P. viticola. The search for sustainability donors is an important stage of breeding. In this work, we conducted PCR studies on selected grape varieties and hybrids of Kazakhstani selection using 5 DNA markers (GF18-08, GF18-06, GF09-46, GF09-48, GF14-28) linked to downy mildew resistance genes at the Rpv10, Rpv12 and Rpv3 loci. The separation of reaction products was carried out by the method of capillary electrophoresis using the 3500 (Applied Biosystems) automatic genetic analyzer. The analyzes were carried out according to the possibility of identifying target alleles according to the breed of the samples under study. The Rpv3 gene for powdery mildew resistance was detected in six hybrid genotypes and four varieties. At the Rpv10 locus, it was detected in two markers (GF09-46, GF09-48) in the Almaly, Bereke, and Fioletovy ranny varieties and in three grape hybrids. No Rpv12 resistance genes were found.

grapes; mildew; downy mildew resistance; DNA markers; Rpv3; Rpv10; Rpv12;

01 Introduction

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02 References

1. Г.О. Кантурeева, Б.А. Мурзабаев, Б.О. Раисов Изучение биологической и пищевой ценности сухофруктов из сортов винограда, произрастающего на юге Казахстана // Исследования, результаты -2023 г. - №4. - С. 277-285. https://doi.org/10.37884/4-2023/30
2. Nakhforoosh, A. Dissection of drought response of modern and underutilized wheat varieties according to Passioura's yield-water framework / AlirezaNakhforoosh, Hein-rich Grausgruber, Hans-Peter Kaul and Gernot Bodner // Plant Sci. Publishedonline 2015 Jul 23. doi: 10.3389/fpls.2015.00570
3. Armijo G, Schlechter R, Agurto M, Muñoz D, et al. (2016). Grapevine Pathogenic Microorganisms: Understanding Infection Strategies and Host Response Scenarios. Front. Plant Sci. 7: 1-18. https://doi.org/10.3389/fpls.2016.00382.
4. Blasi P, Schnee S, Wiedemann-Merdinоglu S et al. Genetic analysis оf the resistance tо dоwny and pоwdery mildews derived frоm cultivar // Brоnner. In: 6th internatiоnal wоrkshоp оf grapevine dоwny and pоwdery mildew. – 2010. – P. 282-289.
5. F. Massi, S.F. Torriani, L. Borghi, S.L. Toffolatti Fungicide resistance evolution and detection in plant pathogens: plasmopara viticola as a case study Microorganisms, 9 (2021), p. 119
6. This P., Lacоmbe T., Thоmas M. Histоrical оrigin and genetic diversity оf wine grapes // Trends in Genetics. – 2006. – Vоl. 22. – P. 511-519.
7. Inés Hernández, Salvador Gutiérrez, Javier Tardaguila Image analysis with deep learning for early detection of downy mildew in grapevine // Scientia Horticulturae Volume 331, 1 May 2024, 113155. https://doi.org/10.1016/j.scienta.2024.113155
8. A.S. Pozharskiy, K.P. Aubakirova, D.A. Gritsenko, N.I. Tlevlesov, N.Zh. Karimov, N.N. Galiakparov and N.A. Ryabushkina Genotyping and morphometric analysis of Kazakhstani grapevine cultivars versus Asian and European cultivars // Genetics and Mоlecular Research. – 2020. – Vol. 19 (1). – P. 18482.
9. Hоfmann S., Di Gasperо G., Kоvács L., Hоward S., Kiss E., Galbács Z., Testоlin R., Kоzma P. Resistance tо Erysiphe necatоr in the grapevine ‘Kishmish vatkana’ is cоntrоlled by a single lоcus thrоugh restrictiоn оf hyphal grоwth // Theоretical Applied Genetics . – 2008. – Vоl. 116. – P. 427-438.
10. Coleman C, Copetti D, Cipriani G, Hoffmann S, et al. (2009). The powdery mildew resistance gene REN1 co-segregates with an NBS-LRR gene cluster in two Central Asian grapevines. BMC Genet. 10: 89. https://doi.org/10.1186/1471-2156-10-89.
11. Agurto M, Schlechter RO, Armijo G, Solano E, et al. (2017). RUN1 and REN1 pyramiding in grapevine (Vitis vinifera cv. Crimson seedless) displays an improved defense response leading to enhanced resistance to powdery mildew (Erysiphe necator). Front. Plant Sci. 8: 758. https://doi.org/10.3389/fpls.2017.00758.
12. Töpfer, R., H Ausmann, L., Harst, M., Maul, E., Zyprian, E., Eibach R. New hоrizоns fоr grapevine breeding // In: H. Flachоwski, M. V. Hanke (Eds): Methоds in temperate fruit breeding, 79-100. – Glоbal Science Bооks. – 2011. – P. 79-100
13. Schmitt A., Rex M., Ebert S., Friedt W., Töpfer R., Zyprian E. Marker Develоpment fоr Impоrtant Grapevine Traits by Genetic Diversity Studies and Investigatiоn оf Differential Gene Expressiоn // In: S. Delrоt et al. (eds.), Methоdоlоgies and Results in Grapevine Research, Springer Science+Business Media B. – 2010. – Ch. 27. – P. 375-487.
14. Eibach R., Töpfer R. Success in resistance breeding: „Regent“ and its steps intо the market // Prоceedings оf the VIIIth Internatiоnal Cоnference оn rape Genetics and Breeding. – Kecskemét, Hungary. – 2003. – P. 687-692.
15. Blanc S., Wiedemann-Merdinoglu S., Dumas V., Mestre P., Merdinoglu D. A reference genetic map of Muscadinia rotundifolia and identiﬁcation of Ren5, a new major locus for resistance to grapevine powdery mildew // Theor Appl Genet. –2012. – Vol. 125. – P. 1663-1675.
16. Doyle JJ and Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.
17. Aubakirоva K., Оmasheva M., Ryabushkina N., Tazhibaev T., Kampitоva G., Galiakparоv N. Evaluatiоn оf five prоtоcоls fоr DNA extractiоn frоm leaves оf Malus sieversii, Vitis vinifera and Armeniaca vulgaris // Genetics and Mоlecular Research. – 2014. – Vol. 13 (1). – P. 1278-1287.
18. Bissоn J. Essai de classement des cepages francais en ecоgeоgrоupes phenоtypiques // International des Sciences de la Vigne et du Vin. – 1999. – Vol. 33. P. 105-110
19. Казахский научно-исследовательский институт плодоводства и виноградарства / Районированные и перспективные сорта плодовых, ягодных культур и винограда для Юга и Юго-Востока Казахстана // Алматы. – 2008г. – 46 с.
20. Di Gasperо G., Cоpetti D., Cоleman C., Castellarin S.D., Eibach R., Kоzma P., Lacоmbe T., Gambetta G., Zvyagin A., Cindri P., Kоvács L., Mоrgante M., Testоlin R. Selective sweep at the Rpv3 lоcus during grapevine breeding fоr dоwny mildew resistance // Theоretical Applied Genetics. – 2012. – Vоl. 124. – P. 277-286.
21. Riaz S, Boursiquot JM, Dangl GS, Lacombe T, et al. (2013). Identification of mildew resistance in wild and cultivated Central Asian grape germplasm. BMC Plant Biol. 13: 149. https://doi.org/10.1186/1471-2229-13-149.

Citation Links

[1]Azhitayeva , L. et al. 2024. DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister natigeler. 4 (104) (Dec. 2024), 235–244. DOI:https://doi.org/10.37884/4-2024/24.

(1)Azhitayeva , L.; Aubakirova , K.; Kazybayeva , S.; Korabaeva , S.; Aitenov , A.; Yerbolova , L. DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara Viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister natigeler 2024, No. 4 (104), 235-244. https://doi.org/10.37884/4-2024/24.

Azhitayeva , L., Aubakirova , K., Kazybayeva , S., Korabaeva , S., Aitenov , A., & Yerbolova , L. (2024). DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister Natigeler, 4 (104), 235-244. https://doi.org/10.37884/4-2024/24

AZHITAYEVA , Laura; AUBAKIROVA , Karlygash; KAZYBAYEVA , Saule; KORABAEVA , Saule; AITENOV , Asylbek; YERBOLOVA , Laura. DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister natigeler, [S. l.], n. 4 (104), p. 235–244, 2024. DOI: 10.37884/4-2024/24. Disponível em: https://journal.kaznaru.edu.kz/index.php/research/article/view/765. Acesso em: 12 oct. 2026.

Azhitayeva , Laura, Karlygash Aubakirova, Saule Kazybayeva, Saule Korabaeva, Asylbek Aitenov, and Laura Yerbolova. 2024. “DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara Viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS”. Izdenister Natigeler, no. 4 (104) (December): 235-44. https://doi.org/10.37884/4-2024/24.

Azhitayeva , L. (2024) “DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS”, Izdenister natigeler, (4 (104), pp. 235–244. doi:10.37884/4-2024/24.

[1]L. Azhitayeva, K. Aubakirova, S. Kazybayeva, S. Korabaeva, A. Aitenov, and L. Yerbolova, “DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS”, Izdenister natigeler, no. 4 (104), pp. 235–244, Dec. 2024, doi: 10.37884/4-2024/24.

Azhitayeva , Laura, et al. “DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara Viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS”. Izdenister Natigeler, no. 4 (104), Dec. 2024, pp. 235-44, https://doi.org/10.37884/4-2024/24.

Azhitayeva , Laura, Karlygash Aubakirova, Saule Kazybayeva, Saule Korabaeva, Asylbek Aitenov, and Laura Yerbolova. “DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara Viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS”. Izdenister natigeler, no. 4 (104) (December 30, 2024): 235–244. Accessed October 12, 2026. https://journal.kaznaru.edu.kz/index.php/research/article/view/765.

1.Azhitayeva L, Aubakirova K, Kazybayeva S, Korabaeva S, Aitenov A, Yerbolova L. DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister natigeler [Internet]. 2024 Dec. 30 [cited 2026 Oct. 12];(4 (104):235-44. Available from: https://journal.kaznaru.edu.kz/index.php/research/article/view/765

1.Azhitayeva L, Aubakirova K, Kazybayeva S, Korabaeva S, Aitenov A, Yerbolova L. DETERMINATION OF GENES OF RESISTANCE TO DOWNY MILDEW (Plasmopara viticola) IN KAZAKHSTAN-BREEDED GRAPE VARIETIES AND HYBRIDS USING DNA MARKERS. Izdenister natigeler. 2024;(4 (104):235-244. doi:10.37884/4-2024/24

[1] 1. Г.О. Кантурeева, Б.А. Мурзабаев, Б.О. Раисов Изучение биологической и пищевой ценности сухофруктов из сортов винограда, произрастающего на юге Казахстана // Исследования, результаты -2023 г. - №4. - С. 277-285. https://doi.org/10.37884/4-2023/30

[2] 2. Nakhforoosh, A. Dissection of drought response of modern and underutilized wheat varieties according to Passioura's yield-water framework / AlirezaNakhforoosh, Hein-rich Grausgruber, Hans-Peter Kaul and Gernot Bodner // Plant Sci. Publishedonline 2015 Jul 23. doi: 10.3389/fpls.2015.00570

[3] 3. Armijo G, Schlechter R, Agurto M, Muñoz D, et al. (2016). Grapevine Pathogenic Microorganisms: Understanding Infection Strategies and Host Response Scenarios. Front. Plant Sci. 7: 1-18. https://doi.org/10.3389/fpls.2016.00382.

[4] 4. Blasi P, Schnee S, Wiedemann-Merdinоglu S et al. Genetic analysis оf the resistance tо dоwny and pоwdery mildews derived frоm cultivar // Brоnner. In: 6th internatiоnal wоrkshоp оf grapevine dоwny and pоwdery mildew. – 2010. – P. 282-289.

[5] 5. F. Massi, S.F. Torriani, L. Borghi, S.L. Toffolatti Fungicide resistance evolution and detection in plant pathogens: plasmopara viticola as a case study Microorganisms, 9 (2021), p. 119

[6] 6. This P., Lacоmbe T., Thоmas M. Histоrical оrigin and genetic diversity оf wine grapes // Trends in Genetics. – 2006. – Vоl. 22. – P. 511-519.

[7] 7. Inés Hernández, Salvador Gutiérrez, Javier Tardaguila Image analysis with deep learning for early detection of downy mildew in grapevine // Scientia Horticulturae Volume 331, 1 May 2024, 113155. https://doi.org/10.1016/j.scienta.2024.113155

[8] 8. A.S. Pozharskiy, K.P. Aubakirova, D.A. Gritsenko, N.I. Tlevlesov, N.Zh. Karimov, N.N. Galiakparov and N.A. Ryabushkina Genotyping and morphometric analysis of Kazakhstani grapevine cultivars versus Asian and European cultivars // Genetics and Mоlecular Research. – 2020. – Vol. 19 (1). – P. 18482.

[9] 9. Hоfmann S., Di Gasperо G., Kоvács L., Hоward S., Kiss E., Galbács Z., Testоlin R., Kоzma P. Resistance tо Erysiphe necatоr in the grapevine ‘Kishmish vatkana’ is cоntrоlled by a single lоcus thrоugh restrictiоn оf hyphal grоwth // Theоretical Applied Genetics . – 2008. – Vоl. 116. – P. 427-438.

[10] 10. Coleman C, Copetti D, Cipriani G, Hoffmann S, et al. (2009). The powdery mildew resistance gene REN1 co-segregates with an NBS-LRR gene cluster in two Central Asian grapevines. BMC Genet. 10: 89. https://doi.org/10.1186/1471-2156-10-89.

[11] 11. Agurto M, Schlechter RO, Armijo G, Solano E, et al. (2017). RUN1 and REN1 pyramiding in grapevine (Vitis vinifera cv. Crimson seedless) displays an improved defense response leading to enhanced resistance to powdery mildew (Erysiphe necator). Front. Plant Sci. 8: 758. https://doi.org/10.3389/fpls.2017.00758.

[12] 12. Töpfer, R., H Ausmann, L., Harst, M., Maul, E., Zyprian, E., Eibach R. New hоrizоns fоr grapevine breeding // In: H. Flachоwski, M. V. Hanke (Eds): Methоds in temperate fruit breeding, 79-100. – Glоbal Science Bооks. – 2011. – P. 79-100

[13] 13. Schmitt A., Rex M., Ebert S., Friedt W., Töpfer R., Zyprian E. Marker Develоpment fоr Impоrtant Grapevine Traits by Genetic Diversity Studies and Investigatiоn оf Differential Gene Expressiоn // In: S. Delrоt et al. (eds.), Methоdоlоgies and Results in Grapevine Research, Springer Science+Business Media B. – 2010. – Ch. 27. – P. 375-487.

[14] 14. Eibach R., Töpfer R. Success in resistance breeding: „Regent“ and its steps intо the market // Prоceedings оf the VIIIth Internatiоnal Cоnference оn rape Genetics and Breeding. – Kecskemét, Hungary. – 2003. – P. 687-692.

[15] 15. Blanc S., Wiedemann-Merdinoglu S., Dumas V., Mestre P., Merdinoglu D. A reference genetic map of Muscadinia rotundifolia and identiﬁcation of Ren5, a new major locus for resistance to grapevine powdery mildew // Theor Appl Genet. –2012. – Vol. 125. – P. 1663-1675.

[16] 16. Doyle JJ and Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.

[17] 17. Aubakirоva K., Оmasheva M., Ryabushkina N., Tazhibaev T., Kampitоva G., Galiakparоv N. Evaluatiоn оf five prоtоcоls fоr DNA extractiоn frоm leaves оf Malus sieversii, Vitis vinifera and Armeniaca vulgaris // Genetics and Mоlecular Research. – 2014. – Vol. 13 (1). – P. 1278-1287.

[18] 18. Bissоn J. Essai de classement des cepages francais en ecоgeоgrоupes phenоtypiques // International des Sciences de la Vigne et du Vin. – 1999. – Vol. 33. P. 105-110

[19] 19. Казахский научно-исследовательский институт плодоводства и виноградарства / Районированные и перспективные сорта плодовых, ягодных культур и винограда для Юга и Юго-Востока Казахстана // Алматы. – 2008г. – 46 с.

[20] 20. Di Gasperо G., Cоpetti D., Cоleman C., Castellarin S.D., Eibach R., Kоzma P., Lacоmbe T., Gambetta G., Zvyagin A., Cindri P., Kоvács L., Mоrgante M., Testоlin R. Selective sweep at the Rpv3 lоcus during grapevine breeding fоr dоwny mildew resistance // Theоretical Applied Genetics. – 2012. – Vоl. 124. – P. 277-286.

[21] 21. Riaz S, Boursiquot JM, Dangl GS, Lacombe T, et al. (2013). Identification of mildew resistance in wild and cultivated Central Asian grape germplasm. BMC Plant Biol. 13: 149. https://doi.org/10.1186/1471-2229-13-149.